Method Validation of Methamphetamine and its Metabolites in Equine Urine

Various precision studies were performed to validate this analysis method using negative control equine urine and standards of the target compounds; methamphetamine, amphetamine, hydroxyamphetamine, hydroxymethamphetamine, and phenylpropanolamine. Initial studies also led to changing the extraction method from liquid-liquid to solid-phase. Once the precision studies were acceptable three independent sets of administered samples obtained from horses dosed with methamphetamine, hydroxyamphetamine, and phenylpropanolamine respectively, were extracted and analyzed using the method. The samples were taken over a range of hours after the dosing, from pre-dose (0 hours) to 48 hours. The method detected all the target compounds except phenylpropanolamine in the methamphetamine set, while the hydroxyamphetamine and phenylpropanolamine sets only had their respective compounds. This research has provided data that demonstrates this method is capable of detecting and quantifying methamphetamine and some of its metabolites in equine urine.