The advancement of biosensors holds significance in swiftly and precisely analyzing biological samples. My research centered on low-dimensional nanomaterials within microfluidic biosensors, aiming for highly sensitive detection. This study is structured into three primary segments. The initial part focuses on the creation of a highly sensitive chemiresistive biosensor utilizing gate-free monolayer graphene (MG). The second part delves into the utilization of a functionalized single-wall carbon nanotube/graphene (FSWCNT/G) composite to craft a high-sensitivity pH sensor. The sensor's performance is assessed by monitoring pH levels in cancer cell solutions through the use of a field-effect transistor (FET), linear sweep voltammetry (LSV), and response time measurement to gauge its effectiveness. The final aspect of the project encompasses a microfluidic pressure sensor based on graphene, which achieved accurate pressure detection.