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Modulation of Alzheimer's Disease Pathology and Neuroplasticity by Environmental Factors

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thesis
posted on 13.12.2012, 00:00 by Yuan-Shih Hu
Alzheimer’s disease (AD) is an aging-linked neurodegenerative disease characterized by memory impairments and cognitive deterioration, and it is associated with a massive loss of neurons in specific brain areas, such as in the hippocampal formation and in the cortex. The pathological hallmarks of the disease include amyloid deposition and neurofibrillary tangles. The familial form of the disease (FAD) is caused by mutations in amyloid precursor protein (APP), presenilin-1 (PS1) or presenilin-2 (PS2). This work suggests that in addition to genetics, environmental factors play an important role in the development and/or progression of the disease. Modulation of environmental factors can promote hippocampal neurogenesis and synaptic plasticity, as well as ameliorates AD pathology. Specifically, we showed that experience of transgenic mice harboring FAD-linked APPswe/PS1ΔE9 in an enriched environment dramatically reduced the levels of oligomeric Aβ and the extent of amyloid deposition in the brains. We also observed a significant reduction in the level of hyperphosphorylated tau concomitantly with the upregulation of the main anterograde motor protein, kinesin-1, in several brain regions of these mice. The molecular pathways underlying the effects of EE on AD pathology and neuroplasticity are not fully understood. In this study, we aimed at investigating the molecular pathways by which environmental enrichment regulates neuroplasticity and tau phosphorylation. We showed that the levels of BDNF and BDNF-associated molecular targets were significantly elevated in the hippocampus following EE, suggesting an increased of BDNF-dependent signaling transductions following EE. In addition, we showed that EE modulated PI3K/Akt/GSK3β signaling pathway in the nontransgenic mice, suggesting that EE induces a reduction in GSK3β activity in an Akt-dependent manner. However, this signaling pathway was not affected in APPswe/PS1ΔE9 mice following EE, suggesting an alternative signaling regulation by EE in FAD mice. Taken together, this work strongly suggests that despite the severe neuropathology, EE can attenuate AD pathology and enhance neuroplasticity in the brains of FAD-linked APPswe/PS1ΔE9 mice. This study further provides several mechanistic explanations for the effects of EE on the AD brains.

History

Advisor

Bongarzone, Ernesto R.

Department

Anatomy and Cell Biology

Degree Grantor

University of Illinois at Chicago

Degree Level

Doctoral

Committee Member

Lazarov, Orly Brady, Scott T. Kessler, John A. Mufson, Elliott J.

Submitted date

2012-08

Language

en

Issue date

13/12/2012