University of Illinois at Chicago

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Nitrate Reductase Activity in Dental Plaque of Children and Adolescents

posted on 2022-08-01, 00:00 authored by Manmit Kaur Sandhu
Nitrate Reductase Activity in Dental Plaque of Children and Adolescents, Sandhu MK, Li W, Kratunova E, Xie, Q, Wu CD, (University of Illinois at Chicago, Chicago, IL) Purpose: Nitrate reductase (NR) of oral bacteria metabolizes dietary nitrate to nitrite, which is further reduced to nitric oxide by gut bacteria and absorbed into blood, thus lowering blood pressure and benefiting cardiovascular health. Limited information is available on NR activity in children. The null hypothesis is that there is no difference in NR activity in dental plaque between children with primary dentition and children with permanent dentition. This study evaluates and compares dental plaque NR activity between children with complete primary dentition and children with complete permanent dentition. Methods: Thirty-four healthy participants (3-15 years old) with primary (N=15) and permanent (N=19) dentitions were enrolled in the study according to specified criteria. One investigator completed dental examination for all participants and collected supragingival plaque from buccal and lingual surfaces of maxillary and mandibular first and second (primary/permanent) molars. Participants’ body mass index (BMI) were recorded. NR activity in plaque samples was assayed using the Griess reagent. Comparison of NR activity between the two groups was performed using the two-sample t-test. Results: NR activity was detected in plaque samples of all participants. However, no statistically significant difference was noted in NR activity between the primary dentition and permanent dentition groups (P=.089). BMI was found to be weakly associated with nitrate reductase activity (R=.384; P=0.027.). Conclusion: While the health benefit of nitrate reductase in children’s oral cavity is not clear, NR activity was detected in children’s dental plaque. Studies regarding the association and contribution of specific microflora and NR activity are warranted.



Wu, Christine


Wu, Christine


Pediatric Dentistry

Degree Grantor

University of Illinois at Chicago

Degree Level

  • Masters

Degree name

MS, Master of Science

Committee Member

Kratunova, Evelina Xie, Qian

Submitted date

August 2022

Thesis type



  • en

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