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Requirement of Myeloperoxidase for Neutrophil Spatial Sensing

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posted on 20.06.2014 by Shalina L. Taylor
Unlike bacteria using a temporal gradient-sensing mechanism, eukaryotic cells sense chemoattractant gradients spatially, that is, they can detect an external gradient as low as 1~2% across a cell diameter. However, the molecular level at which eukaryotic cells achieves spatial sensing remains unknown. Here we report that blood neutrophils migrated faster but completely lost spatially sensing to bacterial formyl peptide gradients when myeloperioxidase (MPO) was knocked out or its activity was inhibited. MPO promoted formyl peptides dissociation from their receptors and refreshed receptors’ capacity to sense external gradients. Therefore, neutrophil directional sensing occurs at the receptor level, prompt dissociation of ligands from their receptors ensures neutrophils migrating precisely toward spatial cues.

History

Advisor

Xu, Jingsong

Department

Pharmacology

Degree Grantor

University of Illinois at Chicago

Degree Level

Doctoral

Committee Member

Minshall, Richard Yuan, Jason de Lanerolle, Primal Ushio-Fukai, Musuko Vogel, Stephen

Submitted date

2014-05

Language

en

Issue date

20/06/2014

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