Solid-State Nuclear Magnetic Resonance of Protein Characterization at Fast Magic Angle Spinning

In the thesis studies, three related projects using SSNMR were carried out on proteins and peptides in solids. In the first project, we implemented high-resolution SSNMR to inspect the secondary structural formation of peptides in the course of SPPS to gain structural insight into the cause of drastically reduced synthesis yield for a long peptide sequence. In the second project, a novel SSNMR approach was established to assist the side-chain signal assignment of trace amount proteins by enhancing both sensitivity and resolution. In the third project, we established a novel SSNMR spectral editing method, termed HIGHLIGHT, to extremely simplify the SSNMR spectra under ultra-fast MAS condition.