System and Method for Precise Positioning a Novel Corneal Electrode in Mice

This thesis presents the development and refinement of a second-generation Multi-Electrode electroretinography (meERG) device tailored for mice, addressing challenges encountered in the first generation. The technology behind the device, including the novel Contact Lens Electrode Array (CLEAr lens) for rats and mice, is explored, with a focus on enhancing data acquisition for experimental research on the mouse eye. The device’s primary aim is the early detection of various retinal diseases, such as retinal detachment, age-related macular degeneration, diabetic retinopathy, and other vision-threatening disorders. Emphasis is placed on the critical role of therapeutic strategies and diagnostic tools, with the Electroretinogram (ERG) standing out for its non-invasiveness. The first-generation CLEAr Lens device prototype began initially developing a downscaled transformation of the rats’ CLEAr Lense in order to incorporate five electrodes for mice. The second-generation device was engineered to overcome challenges in device positioning and alignment. The thesis underscores that the first-generation device encountered testing issues attributable to the protocol for mouse and device positioning, motivating the development of this refined ERG device. The conclusion highlights the potentially transformative impact of this improved technology on enhancing data acquisition in experimental research.