Titanium Dioxide Nanotubes Reinforced Bone Cement Composite: Cytotoxicity And Mechanical Properties
thesisposted on 25.07.2018 by Abhijit H Phakatkar
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This research work provides the novel comparative study between titanium dioxide nanotubes (TNT) and silver-doped hydroxyapatite (Ag-HA) nanobelts used as fillers in the polymethylmethacrylate (PMMA) bone cement matrix. The state of the art PMMA bone cements lack in their sufficient mechanical strength and bioactive characteristics. This study evaluates the mechanical, antibacterial and cytotoxicity properties of PMMA bone cement with the incorporation of TNT and Ag-HA nanobelts at specific weight percent. Individual TNT were synthesized by hydrothermal method. Ag-HA nanobelts were also synthesized by hydrothermal method by assuring the concentration of silver within the allowable non-toxic limit. PMMA bone cement samples were prepared by integrating TNT and Ag-HA nanobelts in the PMMA polymer and methyl methacrylate liquid monomer matrix at specific weight percent. Compression test was performed to evaluate the compressive strength and Young’s modulus for both the fillers. Cytotoxicity of the material was also assessed by (3-(4,5-dimethylthiazol-2-yl)-2,5diphenyl tetrazolium bromide) MTT cells viability assay with 3T3-J2 fibroblast cells line. Additionally, in-vitro antibacterial test was performed by disc diffusion method by using Gram-negative Escherichia Coli bacteria. The results indicated that the compressive strength of the PMMA bone cement was increased with TNT as compared with Ag-HA nanobelts. MTT assay clearly showed that the TNT were not toxic to the cells and Ag-HA nanobelts considerably enhanced cells proliferation by proving the increase in the bioactivity. In-vitro antibacterial test results showed the inhibition zone with the optimum dosage of silver in Ag-HA nanobelts. Hence, TNT and Ag-HA nanobelts can be considered as the substaintial fillers for the existing filler materials for the PMMA bone cement matrix.