Engineering

Process for obtaining nuclear morphometric data, showing a): a Feulgen-stain subimage, one of dozens obtained by breaking up whole slide images scanned at 400× on an Aperio ScanScope-CS®; b): a binary rendition of the previous image, produced by K-means clustering to identify pixels containing DNA; c): the same image with nuclei segmented using watershed algorithms available in Matlab®; d): three-dimensional maps showing optical density for DNA at each pixel in representative benign and PCa nuclei, respectively.

Frequency histograms of multifeature scores (MFS_n) for nuclei from various malignant and benign tissue compartments.

Frequency histograms for MFS_n benign and cancer nuclei from two selected subjects.

Boxplots for population-level multifeature scores (MFS_p) from various tissue compartments in 20 radical prostatectomy subjects and 8 subjects with bladder cancer and supernormal prostates.

Comparing populations of benign nuclei from negative biopsies in a case-control study: subsequent diagnosis of prostate cancer is associated with a “cancer-like” nuclear morphometric score^* (MFSp). Scores derived from external datasets.

A multifeature nuclear morphometric score (MFSp) accurately discriminates cancer vs. benign cell populations: AUC results for two one-step logistic regression models.

Development of a Nuclear Morphometric Signature for Prostate Cancer Risk in Negative Biopsies

The WASP/N-WASP-binding region of EspF_U is required for N-WASP-independent actin pedestal formation.

Pedestal formation on N-WASP-deficient cells requires multiple EspF_U repeats.

WASP/WAVE family members that are not involved in pedestal formation.

The Arp2/3 complex is critical for actin pedestal formation on N-WASP-deficient cells.

EspF_U is necessary and sufficient to drive N-WASP independent pedestal formation.

The Tir-EspF_U linker protein IRTKS localizes to bacteria even in the absence of N-WASP.

Tir_EHEC and EspF_U promote pedestal formation in the absence of N-WASP.

N-WASP is required for efficient intimin-mediated bacterial attachment to the cell surface.

N-WASP-mediated actin assembly facilitates type III translocation of Tir and EspF_U from EHEC.

Enterohemorrhagic E. coli Requires N-WASP for Efficient Type III Translocation but Not for EspF_U-Mediated Actin Pedestal Formation