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dc.contributor.authorBi, Xiuli
dc.contributor.authorQian, Zhibin
dc.contributor.authorYang, George R.
dc.contributor.authorGou, Yuan
dc.contributor.authorGuzman, Grace
dc.contributor.authorBalla, Andre
dc.contributor.authorIozzo, Renato V.
dc.contributor.authorYang, Wancai
dc.contributor.authorPohl, Nicole M.
dc.date.accessioned2013-11-08T15:48:34Z
dc.date.available2013-12-06T19:01:36Z
dc.date.issued2012-02
dc.identifier.bibliographicCitationBi, X. L., Pohl, N. M., Qian, Z. B., Yang, G. R., Gou, Y., Guzman, G., Kajdacsy-Balla, A., Iozzo, R. V., & Yang, W. C. 2012. Decorin-mediated inhibition of colorectal cancer growth and migration is associated with E-cadherin in vitro and in mice. Carcinogenesis, 33(2): 326-330. DOI: 10.1093/carcin/bgr293en_US
dc.identifier.issn0143-3334
dc.identifier.otherDOI: 10.1093/carcin/bgr293
dc.identifier.urihttp://hdl.handle.net/10027/10422
dc.descriptionThis is a pre-copy-editing, author-produced PDF of an article accepted for publication in Carcinogenesis following peer review. The definitive publisher-authenticated version Bi, X. L., Pohl, N. M., Qian, Z. B., Yang, G. R., Gou, Y., Guzman, G., Kajdacsy-Balla, A., Iozzo, R. V., & Yang, W. C. 2012. Decorin-mediated inhibition of colorectal cancer growth and migration is associated with E-cadherin in vitro and in mice. Carcinogenesis, 33(2): 326-330. is available online at: Oxford University Press http://carcin.oxfordjournals.org/content/33/2/326. DOI: 10.1093/carcin/bgr293en_US
dc.description.abstractPrevious studies have shown that decorin expression is significantly reduced in colorectal cancer tissues and cancer cells, and genetic deletion of the decorin gene is sufficient to cause intestinal tumor formation in mice, resulting from a downregulation of p21, p27kip1 and E-Cadherin and an upregulation of β-catenin signaling (Bi et al. Carcinogenesis, 2008). However, the regulation of E-Cadherin by decorin and its implication in cancer formation and metastasis is largely unknown. Using a decorin knockout mouse model (Dcn-/- mice) and manipulated expression of decorin in human colorectal cancer cells we found that E-Cadherin, a protein that regulates cell-cell adhesion, epithelial-mesenchymal transition and metastasis, was almost completely lost in Dcn-/- mouse intestine, and loss of decorin and E-Cadherin accelerated colon cancer cell growth and invasion in Dcn- /- mice. However, increasing decorin expression in colorectal cancer cells attenuated cancer cell malignancy, including inhibition of cancer cell proliferation, promotion of apoptosis, and importantly, attenuation of cancer cell migration. All these changes were linked to the regulation of E-Cadherin by decorin. Moreover, overexpression of decorin upregulated E-Cadherin through increasing of ECadherin protein stability as E-Cadherin mRNA and promoter activity were not affected. Co-immunoprecipitation assay showed a physical binding between decorin and E-Cadherin proteins. Taken together, our results provide direct evidence that decorin-mediated inhibition of colorectal cancer growth and migration is through the interaction with and stabilization of E-Cadherin.en_US
dc.description.sponsorshipThis work was support in part by a grant from the National Cancer Institute, National Institutes of Health, USA (CA112081) and Faculty Startup Fund from the University of Illinois at Chicago (to W.Yang), and a grant from China National Natural Science Foundation (81001003, to X. Bi).en_US
dc.language.isoen_USen_US
dc.publisherOxford University Pressen_US
dc.titleDecorin-mediated inhibition of colorectal cancer growth and migration is associated with E-Cadherin in vitro and in miceen_US
dc.typeArticleen_US


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