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dc.contributor.authorKang, Song Ok
dc.contributor.authorWright, Jordan O.
dc.contributor.authorTesorero, Rafael A.
dc.contributor.authorLee, Hyunwoo
dc.contributor.authorBeall, Bernard
dc.contributor.authorCho, Kyu Hong
dc.date.accessioned2013-11-08T17:19:36Z
dc.date.available2013-11-08T17:19:36Z
dc.date.issued2012-05
dc.identifier.bibliographicCitationKang, S. O., J. O. Wright, et al. (2012). "Theroregulation of Capsule Production by Streptococcus pyogenes." PLoS One 7(5). DOI: 10.1371/journal.pone.0037367en_US
dc.identifier.issn1932-6203
dc.identifier.other10.1371/journal.pone.0037367
dc.identifier.urihttp://hdl.handle.net/10027/10431
dc.description© 2012 Kang et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en_US
dc.description.abstractThe capsule of Streptococcus pyogenes serves as an adhesin as well as an anti-phagocytic factor by binding to CD44 on keratinocytes of the pharyngeal mucosa and the skin, the main entry sites of the pathogen. We discovered that S. pyogenes HSC5 and MGAS315 strains are further thermoregulated for capsule production at a post-transcriptional level in addition to the transcriptional regulation by the CovRS two-component regulatory system. When the transcription of the hasABC capsular biosynthetic locus was de-repressed through mutation of the covRS system, the two strains, which have been used for pathogenesis studies in the laboratory, exhibited markedly increased capsule production at sub-body temperature. Employing transposon mutagenesis, we found that CvfA, a previously identified membrane-associated endoribonuclease, is required for the thermoregulation of capsule synthesis. The mutation of the cvfA gene conferred increased capsule production regardless of temperature. However, the amount of the capsule transcript was not changed by the mutation, indicating that a post-transcriptional regulator mediates between CvfA and thermoregulated capsule production. When we tested naturally occurring invasive mucoid strains, a high percentage (11/53, 21%) of the strains exhibited thermoregulated capsule production. As expected, the mucoid phenotype of these strains at sub-body temperature was due to mutations within the chromosomal covRS genes. Capsule thermoregulation that exhibits high capsule production at lower temperatures that occur on the skin or mucosal surface potentially confers better capability of adhesion and invasion when S. pyogenes penetrates the epithelial surface.en_US
dc.description.sponsorshipThis work was supported by Scientist Development Grant 11SDG7440083 from the American Heart Association.en_US
dc.language.isoen_USen_US
dc.publisherPublic Library of Scienceen_US
dc.titleThermoregulation of Capsule Production by Streptococcus pyogenesen_US
dc.typeArticleen_US


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