HPLC-MTT assay: anti-cancer activity of aqueous garlic extract is from allicin
Jayathilaka, Lasanthi P.
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A strategy using RP-HPLC, TLC, MS, NMR, chemical synthesis, and MTT cell viability assay to identify allicin as the active anti-cancer compound in the aqueous extract of garlic (AGE) is described. Change the pH of the AGE from 7 to 5 eliminated interfering molecules and enabled a clean HPLC separation of the constituents in AGE. MTT assay of the HPLC fractions identify an active fraction. Further analysis by TLC, MS, and NMR verify the active HPLC fraction as allicin. Chemically synthesized allicin is used to provide further confirmation. The results clearly identify the active compound in AGE as allicin.
Aqueous garlic extract
mouse colon cancer cell