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dc.contributor.authorHales, Dale Buchanan
dc.contributor.authorZhuge, Yan
dc.contributor.authorLagman, Jo Ann Jaen
dc.contributor.authorAnsenberger, Kristine
dc.contributor.authorMahon, Cassandra
dc.contributor.authorBarua, Animesh
dc.contributor.authorLuborsky, Judith
dc.contributor.authorBahr, Janice M.
dc.date.accessioned2008-08-28T07:53:02Z
dc.date.available2008-08-28T07:53:02Z
dc.date.issued2008-05-23
dc.identifier.bibliographicCitationHales DB, Zhuge Y, Lagman JA, Ansenberger K, Mahon C, Barua A, Luborsky JL, Bahr JM. Cyclooxygenases expression and distribution in the normal ovary and their role in ovarian cancer in the domestic hen (Gallus domesticus). Endocrine. 2008 May 23. http://www.springerlink.com/content/m48l3534j6w12557/fulltext.pdfen
dc.identifier.issn15599-010
dc.identifier.otherDOI 10.1007/s12020-008-9080-z
dc.identifier.urihttp://hdl.handle.net/10027/2155
dc.descriptionThe original publication is available at www.springerlink.comen
dc.description.abstractCyclooxygenase (COX) (PTGS) is the rate-limiting enzyme in the biosynthesis of prostaglandins. Two COX isoforms have been identified, COX-1 and COX-2, which show distinct cell-specific expression and regulation. Ovarian cancer is the most lethal gynecological malignancy and the disease is poorly understood due to the lack of suitable animal models. The laying hen spontaneously develops epithelial ovarian cancer with few or no symptoms until the cancer has progresses to a late stage, similar to the human disease. The purpose of this study was to examine the relative expression and distribution of COX-1 and COX-2 in the ovaries of normal hens and in hens with ovarian cancer. The results demonstrate that COX-1 was localized to the granulosa cell layer and cortical interstitium, ovarian surface epithelium (OSE) and postovulatory follicle (POF) of the normal ovary. In ovarian cancer, COX-1 mRNA was significantly increased and COX-1 protein was broadly distributed throughout the tumor stroma. COX-2 protein was localized to the granulosa cell layer in the follicle and the ovarian stroma. COX-2 mRNA expression did not change as a function of age or in ovarian cancer. There was significantly higher expression of COX-1 mRNA in the first POF (POF-1) compared to POF-2 and POF-3. COX-2 mRNA expression was not significantly different among POFs. There was no difference in COX-1 or COX-2 mRNA in the OSE isolated from individual follicles in the follicular hierarchy. The results confirm previous findings of the high expression of COX-1 in ovarian tumors further supporting the laying hen as a model for ovarian cancer, and demonstrate for the first time the high expression of COX-1 in POF-1 which is the source of prostaglandins needed for oviposition.en
dc.language.isoen_USen
dc.publisherSpringer Verlagen
dc.subjectOvary Canceren
dc.subjectHensen
dc.subjectCyclooxygenase 1en
dc.subjectCyclooxygenase 2en
dc.titleCyclooxygenases expression and distribution in the normal ovary and their role in ovarian cancer in the domestic hen (Gallus domesticus).en
dc.title.alternativeCOX-1 in normal and cancerous ovaries of the henen
dc.typeArticleen


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