Browsing Publications - Research Resources Center by Title
Now showing items 3-11 of 11
-
Deconstructing the Polymerase Chain Reaction: Understanding and Correcting Bias Associated with Primer Degeneracies and Primer-Template Mismatches
(Public Library of Science, 2015-05-21)The polymerase chain reaction (PCR) is sensitive to mismatches between primer and template, and mismatches can lead to inefficient amplification of targeted regions of DNA template. In PCRs in which a degenerate primer ... -
Denitrifying Bacteria from the Genus Rhodanobacter Dominate Bacterial Communities in the Highly Contaminated Subsurface of a Nuclear Legacy Waste Site
(American Society for Microbiology, 2012-02)The effect of long-term mixed-waste contamination, particularly uranium and nitrate, on the microbial community in the terrestrial subsurface was investigated at the field scale at the Oak Ridge Integrated Field Research ... -
A dynamic noise level algorithm for spectral screening of peptide MS/MS spectra
(BioMed Central, 2010-08-23)Background: High-throughput shotgun proteomics data contain a significant number of spectra from non-peptide ions or spectra of too poor quality to obtain highly confident peptide identifications. These spectra cannot be ... -
Flk1+ and VE-Cadherin+ Endothelial Cells Derived from iPSCs Recapitulates Vascular Development during Differentiation and Display Similar Angiogenic Potential as ESC-Derived Cells
(PLoS One, 2013-12)RATIONALE: Induced pluripotent stem (iPS) cells have emerged as a source of potentially unlimited supply of autologous endothelial cells (ECs) for vascularization. However, the regenerative function of these cells relative ... -
Genome sequences for six Rhodanobacter strains isolated from soils and the terrestrial subsurface with variable denitrification capabilities
(American Society for Microbiology, 2012-08)We report the first genome sequences for six strains of Rhodanobacter species isolated from a variety of soil and subsurface environments. Three of these strains are capable of complete denitrification and three others are ... -
HPLC-MTT assay: anti-cancer activity of aqueous garlic extract is from allicin
(Elsevier Masson, 2013-05)A strategy using RP-HPLC, TLC, MS, NMR, chemical synthesis, and MTT cell viability assay to identify allicin as the active anti-cancer compound in the aqueous extract of garlic (AGE) is described. Change the pH of the AGE ... -
Niacin extended-release/simvastatin combination therapy produces larger favorable changes in high-density lipoprotein particles than atorvastatin monotherapy
(Dove Medical Press, 2012-01-24)The purpose of this research was to compare the effects of niacin extended-release in combination with simvastatin (NER/S) versus atorvastatin monotherapy on high-density lipoprotein (HDL) particle number and size in ... -
Production of antipeptide antibodies
(Humana Press, 2010)Peptides (8-20 residues) are as effective as proteins in raising antibodies, both polyconal and monoclonal with a titer above 20,000 easily achievable. A successful antipeptide antibody production depends on several factors ... -
A ribosomal misincorporation of lys for arg in human triosephosphate isomerase expressed in escherichia coli gives rise to two protein populations
(Public Library of Science, 2011-06-28)We previously observed that human homodimeric triosephosphate isomerase (HsTIM) expressed in Escherichia coli and purified to apparent homogeneity exhibits two significantly different thermal transitions. A detailed ...