Show simple item record

dc.contributor.authorGutka, Hiten J.
dc.contributor.authorRukseree, Kamolchanok
dc.contributor.authorWheeler, Paul R.
dc.contributor.authorFranzblau, Scott G.
dc.contributor.authorMovahedzadeh, Farahnaz
dc.date.accessioned2011-05-27T03:17:13Z
dc.date.available2011-05-27T03:17:13Z
dc.date.issued2011-03-31
dc.identifier.bibliographicCitationGutka, H. J., Rukseree, K., Wheeler, P. R., Franzblau, S. G., & Movahedzadeh, F. 2011. glpX Gene of Mycobacterium tuberculosis: Heterologous Expression, Purification, and Enzymatic Characterization of the Encoded Fructose 1,6-bisphosphatase II. Applied Biochemistry and Biotechnology. DOI: 10.1007/s12010-011-9219-xen
dc.identifier.issn0273-2289
dc.identifier.otherDOI: 10.1007/s12010-011-9219-x
dc.identifier.urihttp://hdl.handle.net/10027/7706
dc.descriptionPost print version of article may differ from published version. The original publication is available at springerlink.com; DOI: 10.1007/s12010-011-9219-x.en
dc.description.abstractThe glpX gene (Rv1099c) of Mycobacterium tuberculosis (Mtb) encodes Fructose 1,6- bisphosphatase II (FBPase II; EC 3.1.3.11); a key gluconeogenic enzyme. Mtb possesses glpX homologue as the major known FBPase. This study explored the expression, purification and enzymatic characterization of functionally active FBPase II from Mtb. The glpX gene was cloned, expressed and purified using a two step purification strategy including affinity and size 2 exclusion chromatography. The specific activity of Mtb FBPase II is 1.3 U/mg. The enzyme is oligomeric, followed Michaelis–Menten kinetics with an apparent km = 44 μM. Enzyme activity is dependent on bivalent metal ions and is inhibited by lithium and inorganic phosphate. The pH optimum and thermostability of the enzyme have been determined. The robust expression, purification and assay protocols ensure sufficient production of this protein for structural biology and screening of inhibitors against this enzyme.en
dc.description.sponsorshipThe authors are thankful to American Lung Association (Grant No. RG-82534-N).en
dc.language.isoen_USen
dc.publisherHumana Pressen
dc.subjectglpXen
dc.subjectFructose 1en
dc.subject6–bisphosphataseen
dc.subjectMycobacterium tuberculosisen
dc.subjectGluconeogenesisen
dc.titleglpX Gene of Mycobacterium tuberculosis: Heterologous Expression, Purification, and Enzymatic Characterization of the Encoded Fructose 1,6-bisphosphatase IIen
dc.typeArticleen


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record