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dc.contributor.authorChang, Lynn
dc.contributor.authorGodinez, William J.
dc.contributor.authorKim, Il-Han
dc.contributor.authorTektonidis, Marco
dc.contributor.authorde Lanerolle, Primal
dc.contributor.authorEils, Roland
dc.contributor.authorRohr, Karl
dc.contributor.authorKnipe, David M.
dc.date.accessioned2012-03-09T19:40:11Z
dc.date.available2012-03-09T19:40:11Z
dc.date.issued2011-05-24
dc.identifier.bibliographicCitationChang, L., Godinez, W. J., Kim, I. H., Tektonidis, M., de Lanerolle, P., Eils, R., Rohr, K., & Knipe, D. M. 2011. Herpesviral Replication Compartments Move and Coalesce at Nuclear Speckles to Enhance Export of Viral Late mRNA. Proceedings of the National Academy of Sciences of the United States of America, 108(21): E136-E144. DOI: 10.1073/pnas.1103411108en
dc.identifier.issn0027-8424
dc.identifier.otherDOI: 10.1073/pnas.1103411108
dc.identifier.urihttp://hdl.handle.net/10027/8182
dc.description© National Academy of Sciences, Proceedings of the National Academy of Sciences of the United States of America. Post print version of article may differ from published version. The definitive version is available through the National Academy of Sciences at DOI: 10.1073/pnas.1103411108.en
dc.description.abstractThe role of the intranuclear movement of chromatin in gene expression is not well understood. Herpes simplex virus forms replication compartments (RCs) in infected cell nuclei as sites of viral DNA replication and late gene transcription. These structures develop from small compartments that grow in size, move and coalesce. Quantitative analysis of RC trajectories, derived from 4D images, show that most RCs move by directed motion. Directed movement is impaired in the presence of actin and myosin inhibitors as well as a transcription inhibitor. In addition, RCs coalesce at and reorganize nuclear speckles. Lastly, distinct effects of actin and myosin inhibitors on viral gene expression suggest that RC movement is not required for transcription but rather, movement results in the bridging of transcriptionally active RCs with nuclear speckles to form structures that enhance export of viral late mRNAs.en
dc.description.sponsorshipWe thank the New England Regional Center for Excellence for Biodefense 26 and Emerging Infectious Diseases (National Institutes of Health Grant AI057159) imaging facility at the Immune Disease Institute for use of the spinning disk confocal microscope. Funding for this work was provided by NIH grant AI63106 to DMK and RO1GM080587 to PdL. Support for the project VIROQUANT (0313923) by the German Federal Ministry of Education and Research (BMBF) (FORSYS) is also acknowledged.en
dc.language.isoen_USen
dc.publisherNational Academy of Sciencesen
dc.subjectsimplex virus infectionen
dc.subjectsingle particle trackingen
dc.subjectDNA binding proteinen
dc.subjectbeta globin locusen
dc.titleHerpesviral Replication Compartments Move and Coalesce at Nuclear Speckles to Enhance Export of Viral Late mRNAen
dc.typeArticleen


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