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dc.contributor.authorDakshinamoorthy, Gajalakshmi
dc.contributor.authorSamykutty, Abhilash Kumble
dc.contributor.authorMunirathinam, Gnanasekar
dc.contributor.authorShinde, Gangadhar Bhaurao
dc.contributor.authorNutman, Thomas
dc.contributor.authorThomas, Maryada Venkatarami
dc.contributor.authorKalyanasundaram, Ramaswamy
dc.date.accessioned2012-08-14T18:35:39Z
dc.date.available2012-08-14T18:35:39Z
dc.date.issued2012-04
dc.identifier.bibliographicCitationDakshinamoorthy, G., Samykutty, A. K., Munirathinam, G., Shinde, G. B., Nutman, T., Reddy, M. V., & Kalyanasundaram, R. 2012. Biochemical Characterization and Evaluation of a Brugia malayi Small Heat Shock Protein as a Vaccine against Lymphatic Filariasis. PLoS One, 7(3): e34077. doi:10.1371/journal.pone.0034077en
dc.identifier.issn1932-6203
dc.identifier.otherdoi:10.1371/journal.pone.0034077
dc.identifier.urihttp://hdl.handle.net/10027/8450
dc.description© 2012 Dakshinamoorthy et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. doi:10.1371/journal.pone.0034077en
dc.description.abstractFilarial nematodes enjoy one of the longest life spans of any human pathogen due to effective immune evasion strategies developed by the parasite. Among the various immune evasion strategies exhibited by the parasite, Interleukin 10 (IL-10) productions and IL-10 mediated immune suppression has significant negative impact on the host immune system. Recently, we identified a small heat shock protein expressed by Brugia malayi (BmHsp12.6) that can bind to soluble human IL-10 receptor alpha (IL-10R) and activate IL-10 mediated effects in cell lines. In this study we show that the IL-10R binding region of BmHsp12.6 is localized to its N-terminal region. This region has significant sequence similarity to the receptor binding region of human IL-10. In vitro studies confirm that the N-terminal region of BmHsp12.6 (N-BmHsp12.6) has IL-10 like activity and the region containing the alpha crystalline domain and C-terminus of BmHsp12.6 (BmHsp12.6αc) has no IL-10 like activity. However, BmHsp12.6αc contains B cell, T cell and CTL epitopes. Members of the sHSP families are excellent vaccine candidates. Evaluation of sera samples from putatively immune endemic normal (EN) subjects showed IgG1 and IgG3 antibodies against BmHsp12.6αc and these antibodies were involved in the ADCC mediated protection. Subsequent vaccination trials with BmHsp12.6αc in a mouse model using a heterologous prime boost approach showed that 83% protection can be achieved against B. malayi L3 challenge. Results presented in this study thus show that the N-BmHsp12.6 subunit of BmHsp12.6 has immunoregulatory function, whereas, the BmHsp12.6αc subunit of BmHsp12.6 has significant vaccine potential.en
dc.description.sponsorshipThis study was funded by an NIH RO1 grant (AI064745). Additional funding was received from the Department of Biotechnology, Ministry of Science and Technology, New Delhi (BT/INF/22/1/2007). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.en
dc.language.isoen_USen
dc.publisherPublic Library of Scienceen
dc.titleBiochemical Characterization and Evaluation of a Brugia malayi Small Heat Shock Protein as a Vaccine Against Lymphatic Filariasisen
dc.typeArticleen


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