Show simple item record

dc.contributor.advisorCho, Michael R.en_US
dc.contributor.authorShafiq, Maryam A.en_US
dc.date.accessioned2013-02-21T21:08:00Z
dc.date.available2013-02-21T21:08:00Z
dc.date.created2012-12en_US
dc.date.issued2013-02-21
dc.date.submitted2012-12en_US
dc.identifier.urihttp://hdl.handle.net/10027/9721
dc.description.abstractIn this project, we strived to develop a decellularized human cornea to use as a scaffold for reconstructing the corneal epithelium and anterior stroma. Human cadaver corneas were decellularized by five different methods, including detergent- and nondetergent-based approaches. The success of each method on the removal of cells from the cornea was investigated. The structural integrity of decellularized corneas was compared with the native cornea by electron microscopy. The integrity of the basement membrane of the epithelium was analyzed by histology and by the expression of collagen type IV, laminin, and fibronectin. Finally, the ability of the decellularized corneas to support the growth of human corneal epithelial cells and fibroblasts was assessed in vitro. Corneas processed using Triton X-100, liquid nitrogen, and poly(ethylene glycol) resulted in incomplete removal of cellular material. Corneas processed with the use of sodium dodecyl sulfate (SDS) or with sodium chloride (NaCl) plus nucleases successfully removed all cellular material; however, only the NaCl plus nuclease treatment kept the epithelial basement membrane completely intact. The elastic modulus between the NaCl decellularized cornea and the native cornea (control) showed no significant difference compared by the Student’s t-test. Corneas processed with NaCl plus nuclease supported both fibroblast and epithelial cell growth in vitro. For in vivo study, NaCl decellularized graft was transplanted on a rat cornea ablated with excimer laser showed graft epithelialization as demonstrated by a reduction in flourescein staining over time. In summary, this is the first study on decellularization of human cornea with which recellularization with both corneal epithelial cells and fibroblasts was demonstrated. The novel method for decellularization has potential applications in corneal and limbal tissue engineering, where a biopsy may be used to grow both epithelial cells and stromal cells to reconstruct the corneal epithelium with a stromal niche.en_US
dc.language.isoenen_US
dc.rightsen_US
dc.rightsCopyright 2012 Maryam A. Shafiqen_US
dc.subjectDecellularizationen_US
dc.subjectCorneal Engineeringen_US
dc.subjectStromaen_US
dc.subjectEpithelial Stem Cellsen_US
dc.subjectLimbal Engineeringen_US
dc.subjectKeratocytesen_US
dc.subjectReconstruction of Corneal Epitheliumen_US
dc.titleDecellularized Human Cornea for Reconstructing the Corneal Epithelium and Anterior Stromaen_US
thesis.degree.departmentBioengineeringen_US
thesis.degree.disciplineBioengineeringen_US
thesis.degree.grantorUniversity of Illinois at Chicagoen_US
thesis.degree.levelDoctoralen_US
thesis.degree.namePhD, Doctor of Philosophyen_US
dc.type.genrethesisen_US
dc.contributor.committeeMemberDjalilian, Ali R.en_US
dc.contributor.committeeMemberEddington, David T.en_US
dc.contributor.committeeMemberYue, Beatrice Y.en_US
dc.contributor.committeeMemberGemeinhart, Richard A.en_US
dc.type.materialtexten_US


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record