Biological Assessment of Used Dental Implant Healing Abutments
thesisposted on 2020-05-01, 00:00 authored by Aniruddh Narvekar
Objective: To determine if healing abutments (HA) can be truly “decontaminated” and to evaluate 4 decontamination strategies, available in most clinical settings, to determine the extent to which biomaterial can be removed compared to new unused HA. Methods: Forty HA were collected from subjects with prior implant placement were randomly distributed and detoxified using four protocols (A-D, N=10/group). Residual protein quantification (N=3/group) and macroscopic debris evaluation (N=2/group) was assessed. Human primary monocyte-derived macrophages (Mφ) were challenged with HA (N=5/group) and supernatants were collected at 4, 24, 48 and 120 h to analyze secretion of cytokines/chemokines. Results: Our study showed test groups (A-D) were not able to be “decontaminated” to levels of new, unused HA. A multiplex analyte assay performed on “decontaminated” test groups (A-D) revealed high levels of cytokine and chemokine secretion. HA should not be re-used due to the potential to initiate an inflammatory response, even after “decontamination” procedures are utilized.
Degree GrantorUniversity of Illinois at Chicago
Degree nameMS, Master of Science
Committee MemberNaqvi, Afsar Tozum, Tolga Ashrafi, Seema Yuan, Judy
Submitted dateMay 2020